Purification of recombinant proteins by chemical removal of the affinity tag
نویسندگان
چکیده
منابع مشابه
Purification of Tag-Free Recombinant Proteins Using the Profinity eXact Fusion-Tag System affinity purification
Introduction Affinity tagging has become a popular strategy for purifying recombinant proteins or functional domains in the postgenomic era. Fusing recombinant proteins with an amino acid sequence that selectively interacts with ligands on chromatographic matrices allows purification of virtually any protein without prior knowledge of its physical properties or physiological activities. This is...
متن کاملAffinity Based Nano-Magnetic Particles for Purification of Recombinant Proteins in Form of Inclusion Body
Background: Protein purification is the most complicated issue in the downstream processes of recombinant protein production; therefore, improved selective purification methods are important. Affinity-based protein purification method using His-tag and Ni-NTA resins is one of the most common strategies. MNPs can be used as a beneficial alternative for Ni-NTA resins. However, there is no data on...
متن کاملCurrent strategies for the use of affinity tags and tag removal for the purification of recombinant proteins.
Affinity tags are highly efficient tools for protein purification. They allow the purification of virtually any protein without any prior knowledge of its biochemical properties. The use of affinity tags has therefore become widespread in several areas of research e.g., high throughput expression studies aimed at finding a biological function to large numbers of yet uncharacterized proteins. In...
متن کاملRecombinant production and affinity purification of the FraC pore forming toxin using hexa-His tag and pET expression cassette
Objective(s): A newly-introduced protein toxin from a sea anemone, namely fragaceatoxin C is a protein with molecular weight of 20 kDa and pore-forming capability against cell membranes has recently grasped great attentions for its function. In this study, its coding sequence cloned as a fusion protein with His-tag for simple production and rapid purification. Materials and Methods: After PCR a...
متن کاملSynthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
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ژورنال
عنوان ژورنال: Applied Biochemistry and Biotechnology
سال: 1998
ISSN: 0273-2289,1559-0291
DOI: 10.1007/bf02787176